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KMID : 0382420030290040021
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Korean Journal of Environmental Health Society
2003 Volume.29 No. 4 p.21 ~ p.26
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Non-invasive Biological Monitoring of DNA Adducts Formed at Workers Handling 3,3’ -Dichlorobenzidine(DCB) by Using GC/MS
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Lee Jin-Heon
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Abstract
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We examine the metabolites(DCB and acetyl DCB) extracted from exfoliated urothelial cells of 33 workers who employed DCB-handling industries. The characteristics of workers submitted urine. whose age. working years and smoking persons were 41.9±11.1, 8.7±5.5 and 25(32.0%). respectively. DNA adduct was isolated from the exfoliated urothelial cells by applying ₃₂p-postlabeling procedure. Metabolites(DCB and acetyl DCB) were extracted from DNA adducts by hydrolyzing and N-glycosylase. Concentrations of DCB and acetyl DCB were 28.6± 5.25 ng/g DNA. and 17.0±3.73 ng/g DNA, respectively. The regression between DCB level and exposure years of workers is y=1.668+2.588×(p=0.005, r₂=0.394). The regression between acetyl DCB level and exposure years of workers is y=8.071+1.325×(p=0.076, r₂=0.222). Smoking workers are significantly higher than non-smoking workers on DCB and acetyl DCB level(p=0.065 and 0.021. respectively). DCB level was 33.9±7.14 ng/g DNA on smokers, and 23.1±9.97 ng/g DNA on non-smokers. Acetyl DCB was 25.1±5.27 nglg DNA on smokers, and 8.92±7.22 ng/g DNA on non-smokers.
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